A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). 1. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. S. 340 PC384. 00. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. Step 4: Incubate at room temperature for 1 hour, protected from light. The plate reader optics are tunable for absorbance,. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Fill a clean plate with water. ] Example of document to evaluate if the reader . When connecting for the first time to either the SpectraMax L reader or StakMax system. Turn the reader on and secure the USB cable. 384 deep well plate: packed in sleeves of 6 , 24 per case. ABS2 Validation Plate Protocol Installation InstructionsEndpoint, dual read, kinetic, fast kinetic. System setup and calibration are controlled from within SoftMax Pro Software. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. V. 561. Assay flexibility. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 0 . Laboratory automation workflows can help. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. FLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 0200-6262. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Microplates for applications that require high sample recovery rates. One plate carrier adapter is provided with the instrument. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Fluorescence Microplate Reader Comparison Chart. 5 µL of each sample was pipetted into a 96-well. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemPrimary considerations when choosing a microplate reader are throughput and flexibility. Single-mode Microplate Reader. 2. Molecular Devices' microplate readers. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. The Beacon Melamine Plate Kit is a competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of melamine in contaminated samples. Table 1: FLIPR hardware settings for a cell plate basal fluorescence signal test. Figure 2. . M2. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. ISM Equipment Classification (Group 1, Class A) This equipment is. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated— any potential issues are. Load up the plates that you need to run and walk away. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535. 0. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. Search. Instrument settings can be saved as a protocol file. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. If the computer is connected to the Internet: Select Search Automatically for. Integrated to. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. 0 software Download page. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. ORDER. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. +44-118-944-8000. Eight filter options provide simplified assay setup. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofRead plate standard and mix. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. User selects appropriate wells for samples and control using plate configuration tool. Sale price $18,499. (a) Chemical structures of 1 (HADP) and its analogues 2–4. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. • Analyze data in a single plate, across multiple plates, or between different groups. . One plate carrier adapter is provided with the instrument. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. Firmly grasp the snap connector beside the snap, then push the snap down. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. 313 reference gain check fail. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Installation Qualification (IQ)the iD5 reader’s filter system. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Select Update Driver. Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. StakMax Handling Stacker Base Unit. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Step 2: Launch SoftMax Pro Software. 25 to 3. Molecular Devices microplate readers. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. These plate readers in the list below, are no longer included with the new SoftMax Pro 6 reader menu. Turn the reader on and secure the USB cable. 76358-624. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. adipocytes: insulin dose-response. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. FlexStation® 3 Multi-Mode Microplate Reader. Timothy Bolus, MPM, MBA, Compliance Program Manager. Plate section without PathCheck applied. BMG Labtech - THERMOstar. Figure 1. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. 1-1. ** Some compatible filter sets are available for purchase from Life. #4TI-0655) Microplates. 5577 UK +44. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. W×D×H. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. Flexibility to read a variety of plate formats. 6% for the on-board optics (monochromators). Absorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. This can increase throughput and efficiency, thus saving time and space in the lab. Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µl. The plate was placed on a shaker for 30 seconds and then incubated for 3 hours at 37°C, 5% CO 2. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. IMAP assays are based on binding of. Superior optical system includes a xenon flash lamp and features. An industry-renowned instrument for monitoring GPCRs and ion channels. These lab workhorses are used in drug discovery. Category 2 and 3 microplate readers use gradient heating as well. +1 800-635-5577. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Fluorescence Microplate Reader Comparison Chart. Fluorescence Microplate Reader Comparison Chart. 944. in steps of 0. Table 2 summarizes the results for the uniformity test. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Fluorescence Microplate Reader Comparison Chart. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Page 17 Chapter 2: Setting Up the Instrument 4. This process should take about 3 - 5 minutes. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. Published Dec 16, 2020 | Updated Dec 16, 2020. “Sensitivity is pretty much clutch for luminescence,” says Daly. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 1. 5: Schematic of the back panel of a reader. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. 400×320×350 mm. Supersedes 1. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Assay flexibility. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Molecular Devices' microplate readers. The CellTiter-Glo assay from Promega uses luciferase. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. S. SpectraMax Microplate Readers. Clone. 3 foot) See. SoftMax Pro 7. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. 2. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. This technical note is designed to provide information . Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution for . Select Update Driver. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Page 28 Chapter 5: Read Modes and Read Types 1. SoftMax Pro GxP Software. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Find regional support. The luminescent reaction must be very intense for a reliable reading. Step 2. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. We would like to show you a description here but the site won’t allow us. F3/F5 Multi Mode Microplate Readers. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Control shake mode, duration, and intensity for a variety of plate formats. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. performs within its specifications. 5 minutes (ValitaTiter) 15 minutes (ValitaTiter PLUS) Integrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 40266 Email: drugdiscoverytech@lifetech. Select Start>Control panel>Device Manager. The computer controlling the plate reader runs Windows XP. In the M2, M5 and Plus. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Published Dec 16, 2020 | Updated Dec 06, 2021. Microplate Reader, Multimode Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Versa. Sunnyvale, CA - May 17, 2011 - Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax® M5e Multi-Mode Microplate Reader for use in NASA's International Space Station; the M5e Reader will be the first. AXON Patch-Clamp. Three low volume 24-well bottom slides. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader. Molecular Devices Software. Page 16 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide To exchange an excitation or emission filter in a slide: 1. A standard spectrophotometer measures the absorbance of one sample at a time. 425 or. Products : General, FilterMax F5 reader. Page 1 of 8 . 0 . This can increase throughput and efficiency, thus saving time and space in the lab. Microplate Readers. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 1. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Step 1: Capture antibody binds to ELISA plate wells. plate readers. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Microplate Reader, Multimode. Microplate Stacker and Washer. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. To update the driver. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. Absorbance Plate Reader Comparison Chart. Turn on Plate Reader and wait for it to complete its start-up routine. It consists of a SpectraMax® Microplate Reader, Peak Robotics S-LAB Plate Handler with the S-LAB software, and the AquaMax® 4000 Microplate Washer. This can increase throughput and efficiency, thus saving time and space in the lab. 0%, raw data units = counts/sec. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and. Fluorescence Microplate Reader Comparison Chart. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . No accessories are needed and SoftMax® Pro Software. Support. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Microplate Readers. 5 sec. The low profile increases robotic stack capacity and storage. together with a Molecular Devices plate. 150 μL of MTT solvent was then added to each well to solubilize the formazan product, and the plate was placed on a shaker for 15 minutes. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. We would like to show you a description here but the site won’t allow us. 96 deep well plate: packed in sleeves of 6 , 24 per case. Step 1: Open the SoftMax Pro IMAP FP protocol. #iD3) Methods. The plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and mixed. NOT FOR USE IN DIAGNOSTIC PROCEDURES. the iD5 reader’s filter system. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. SoftMax® Pro 7. 2. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Instrument settings can be saved as a protocol file. What are the physical and environmental operating specifications for SpectraMax UV/Vis and Gemini plate readers? (With extra info on backup power UPS choices). Microplate Readers. Test up to 3. a single plate read using any standard cuvette, or 96- or 384-well microplate. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 1. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. One plate carrier adapter is provided with the instrument. EMax. With optimized reagents, option to operate the. Every step is optimized to simplify analysis and reporting to support our microplate readers. 1). Molecular Devices provides web-based tutorials (webinars) and training courses throughout the year. Lab automation for high-throughput plate-based assays. When the user applies a set temperature of 37°C, the top is heated to 37. #S1205. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. Download PDF. ” So, acquisition and analysis come from one software system. 7°C and the bottom to 36. 24 deep well. SoftMax Pro Software. First, as mentioned before, the walkaway time significantly. ®This document is provided to customers who have purchased Molecular Devices, Inc. Transfected cells in the 6-well plate were trypsinized, divided into ten aliquots per well, pelleted at 1500 rpm for 5 minutes, and washed once with PBS. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. Absorbance Plate Reader Comparison Chart. Multi-mode Plate Reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. 1. This offer includes SpectraMax i3x, SpectraMax. Instrument settings can be saved as a protocol file. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the. 2. The Versamax Microplate Reader is built around a grating monochromator. Read one sample or up to 384 in a. begin reading microplates with seven mouse clicks. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Beyond the hardware, a microplate reader’s software also impacts performance and flexibility. The Back Panel Figure 1. San Jose, California, June 5, 2018. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. The drawing shows a standard microplate of 15 mm height covered with a lid. Molecular Devices Software. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. 0) OD (500 to 1100) RFU (5. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Cell viability는 세포막 Integrity 또는. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Cellular Imaging Systems. Toggle navigation 0 Cart; Store. SpectraMax Readers have the most complete level of product validation and compliance. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. 2. Restart the system. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. They feature options and modules to address your specific research including objectives, filters, imaging modes, and environmental. To interrupt the kinetic measurement, the. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. GxP. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Tests every subsystem of the reader . Our extensive SpectraMax® line of user-friendly microplate readers – the. #Flex3) Methods. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 997 (n = 3). 2. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. 9. , prior to compound addition) from the cells. in steps of 0. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners.